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The cells were grown in Endothelial Cell Medium (ECM, ScienCell Research Laboratories, Carlsbad, CA, USA) supplemented with 10% FBS, 1% (v/v) penicillin/streptomycin, and 1% endothelial cell growth factors at 37 C with 5% CO 2 and 95% air

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Using Virtual AuthTech or iVECoder allows payers and providers to compare reimbursement at different Medicare percentages, evaluate state-by-state differences via GPCI, and identify when contracted rates fail to reflect true service value